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. 2017 Aug 4;12(8):e0180711. doi: 10.1371/journal.pone.0180711

Fig 6. Effect of Wnt5a and Rspo-2 treatments on non-canonical Wnt pathway signaling in normal and OA osteoblasts.

Fig 6

Confluent normal and OA osteoblasts were treated with rhWnt5a, rhRspo2 or both for 15 minutes. Proteins were prepared from cells for Western blot analysis. A) Representative Western blot analysis of normal and OA osteoblasts; B) Representative Western blot analysis for phospho-PKC and phospho-JNK of OA osteoblasts treated with siWnt5a or a siSCR (control); C) Quantification of the phosphorylation of JNK (p-JNK) in normal and OA osteoblasts relative to actin loading (OA: n = 5, normal: n = 3); D) Quantification of the phosphorylation of PKC (p-PKC) in normal and OA osteoblasts relative to actin loading (OA: n = 4 and N: n = 3); E) NFAT activity level measured with dual-luciferase reporter assay following rhWnt5a treatment in OA osteoblasts (n = 8); F) AP-1 activity level measured with dual-luciferase reporter assay following rhWnt-5a treatment in OA osteoblasts (n = 3); G) TOPflash activity level in response to Wnt3a, Wnt5a or both in OA osteoblasts (n = 4).