Table 1. Primer sequences and amplification summary.
Gene | Symbol | Reference | 5'-3' sequence | Amplicon length (pb) |
---|---|---|---|---|
Glutamate decarboxylase 2 | Gad65/Gad2 | NM_012563.1 | F-CAATGTTCGGCTCTCCTGGT | 120 |
R-CTTGTCTCCCGTGTCATAGG | ||||
Glutamate decarboxylase 1 | Gad67/Gad1 | NM_017007.1 | F-ATCCTAATACTACCAACCTGC | 55 |
R-GCTACGCCACACCAAGTATCA | ||||
Glial fibrillary acidic protein | Gfap | NM_017009.2 | F-AACCGCATCACCATTCCTGT | 123 |
R-CATCTCCACCGTCTTTACCAC | ||||
Neuropeptide Y | Npy | NM_012614.2 | F-GCTCTGCGACACTACATCAATC | 147 |
R-CCATCACCACATGGAAGGGT | ||||
Peptidylprolyl isomerase A (cyclophilin A) | Ppia | NM_017101.1 | F- GGTCCTGGCATCTTGTCCAT | 134 |
R-GCCTTCTTTCACCTTCCCAA | ||||
Tumor necrosis factor | Tnf | NM_012675.3 | F-GCTCCCTCTCATCAGTTCCA | 106 |
R-CTCCGCTTGGTGGTTTGCTA | ||||
Actin beta | Actb | NM_031144.3 | F-AGCCTTCCTTCCTGGGTA | 92 |
R-GAGGTCTTTACGGATGTCAAC |