Table 1. INCLUSIVITY in Trypanosoma cruzi strains representative of different DTUs (fg/test).
Tc I (Sylvio X10) | 1.0 x 10−1 | 1.0 x 10−2 | 1.0 x 10−3 | 1.0 x 10−4 |
Naked eye detection | +/- | + /- | -/- | -/- |
Real Time mean Ct× | 23.25 | 27.8 | NDt/NDt | NDt/NDt |
Tc II (JG1) | 2.5 | 2.5 x 10−1 | 2.5 x 10−2 | 2.5 x 10−3 |
Naked eye detection | +/+ | -/- | -/- | -/- |
Real Time mean Ct | 13.47 | NDt/NDt | NDt/NDt | NDt/NDt |
Tc III (M6241) | 7.5 x 10−1 | 7.5 x 10−2 | 7.5 x 10−3 | 7.5 x 10−4 |
Naked eye detection | +/+ | +/- | -/- | -/- |
Real Time mean Ct | 8.68 | 11.81/NDt | NDt/NDt | NDt/NDt |
Tc IV (M4167) | 5.0 | 5.0 x 10−1 | 5.0 x 10−2 | 5.0 x 10−3 |
Naked eye detection | +/+ | +/+ | +/- | -/- |
Real Time mean Ct | 8.66 | 11.88 | 12.74/NDt | NDt/NDt |
Tc V (PAH179) | 1.5 | 1.5 x 10−1 | 1.5 x 10−2 | 1.5 x 10−3 |
Naked eye detection | +/+ | +/+ | -/- | -/- |
Real Time mean Ct | 10.20 | 12.98 | NDt/NDt | NDt/NDt |
Tc VI (CL-Brener) | 1.0 | 1.0 x 10−1 | 1.0 x 10−2 | 1.0 x 10−3 |
Naked eye detection | +/- | +/- | -/- | -/- |
Real Time mean Ct | 12.68 | 25.24 | NDt/NDt | NDt/NDt |
. NDt: Non detectable. X When only one replicate was LAMP positive, the Ct of that sample was reported.