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PC-3 cells were incubated with LPS (2.0 μg/mL) in absence or presence of TGP (312.5 μg/mL). Cells and cultural supernatants were collected at 6 hr after LPS. (a) IL-6 and (c) IL-8 mRNAs were determined using RT-PCR. (b) IL-6 and (d) IL-8 in culture medium were measured using ELISA. Data were expressed as means ± S.E.M. (N = 6), **P<0.01.
