Table S1.

RT-qPCR–based validation of microarray experiments

		Fold change transcription		P value transcription		Fold change polysome		P value polysome		Transcription (qPCR)	Translation
Probe	Gene	Array	qPCR	Array	qPCR	Array	qPCR	Array	qPCR	Down in Lck−/−	Down in Lck−/− (array)	Down in Lck−/− (qPCR)
A_55_P1980796	Il2ra (CD25)	−1.32	−1.29	0.006	0.431	−3.57	−2.80	0.001	0.041	No	Yes	Yes
A_51_P181286	CD69	1.04	1.25	0.218	0.652	−1.81	−1.27	0.264	0.181	No	No	No
A_55_P1974877	BclxL	1.05	1.27	0.282	0.461	−2.86	−3.14	0.008	0.005	No	Yes	Yes
A_52_P1197913	Gadd45b	1.52	1.58	0.779	0.359	−2.86	−2.29	0.011	0.002	No	Yes	Yes
A_52_P108346	Myc	1.14	1.06	0.779	0.847	−2.02	−3.70	0.138	0.024	No	No	Yes
A_52_P99848	Pik3cd	1.37	−1.11	0.994	0.794	−2.21	−1.64	0.088	0.043	No	No	Yes
A_52_P68893	IFNg	1.47	2.11	0.203	0.217	−2.81	−2.32	0.018	0.004	No	Yes	Yes
A_51_P391445	Ifngr1	1.52	1.11	0.934	0.716	−2.46	−1.44	0.050	0.012	No	Yes	Yes
A_51_P286496	Il2rb	1.31	−1.24	0.998	0.413	−2.87	−3.40	0.009	0.029	No	Yes	Yes
A_51_P470715	Cish	1.51	−1.00	0.998	0.995	−2.91	−4.74	0.009	0.028	No	Yes	Yes
A_51_P279606	Socs1	1.63	−1.14	1.000	0.717	−1.59	−1.62	0.355	0.089	No	No	No
A_51_P474459	Socs3	1.78	−0.97	1.000	0.929	−1.87	−6.54	0.252	0.024	No	No	Yes
A_55_P2085157	Eef1g	1.07	1.01	0.975	0.986	−1.30	−1.19	0.477	0.478	No	No	No
A_66_P106191	Eef2	1.06	−1.34	0.882	0.305	−1.98	−1.86	0.204	0.225	No	No	No
A_55_P2128451	Sfpq	−1.00	1.19	0.398	0.748	−2.45	−1.97	0.037	0.010	No	Yes	Yes

Total transcript abundance and polysome:subpolysome ratio of 15 genes involved in T-cell activation, signaling pathways, effector function, and survival were measured by quantitative PCR using the same biological samples as those used in the microarrays (n = 4, two-way t test, P value threshold 0.05). Validated significant P values are highlighted in bold.