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Triple silencing of NRC2, NRC3, and NRC4 compromised Rx-mediated extreme resistance to PVX. NRC2, NRC3, or NRC4 was silenced individually or in combination in Rx transgenic plants by TRV. SGT1 silencing, which compromises Rx-mediated resistance, was used as a control. The circles on the inoculated leaves indicate the area of PVX inoculation by agroinfection. Photographs were taken 2 wk after PVX inoculation.
