Table S1.

Number of dA3 derivatives (NTS, area postrema, A1/C1, A2/C2 neurons) and calls in individual TxPh-1 and TxPh-2 mutant animals

Genotype	dA3 neuronal derivatives, n				Calls
	NTS neurons	AP neurons	A2/C2 neurons	A1/C1 neurons	Mechanical stimulation		Isolation, USCs, n
					ACs, n	USCs, n
WT 1	17,144	2,947	621	841	3	8	36
WT 2	16,891	2,781	579	785	2	5	40
WT 3	15,997	2,877	584	794	4	10	58
WT 4	17,581	2,864	548	765	3	5	35
WT 5	16,985	2,854	601	763	3	8	37
WT 6	17,304	2,994	586	789	5	8	40
WT 7	ND	ND	ND	ND	8	8	41
WT 8	ND	ND	ND	ND	6	6	38
WT 9	ND	ND	ND	ND	6	12	36
WT 10	ND	ND	ND	ND	5	8	38
WT 11	ND	ND	ND	ND	5	9	37
TxCon 1	16,988	2,674	611	712	7	9	55
TxCon 2	16,995	2,596	599	852	6	8	54
TxCon 3	17,288	2,892	614	764	8	7	44
TxCon 4	16,788	2,955	568	729	4	6	35
TxCon 5	16,459	2,711	674	781	3	12	34
TxCon 6	16,598	2,859	626	728	3	8	27
TxCon 7	ND	ND	ND	ND	4	8	4
TxCon 8	ND	ND	ND	ND	5	7	58
TxCon 9	ND	ND	ND	ND	5	12	37
TxCon 10	ND	ND	ND	ND	4	11	22
TxCon 11	ND	ND	ND	ND	5	10	27
TxCon 12	ND	ND	ND	ND	4	8	22
TxCon 13	ND	ND	ND	ND	3	7	24
TxCon 14	ND	ND	ND	ND	8	9	22
TxCon 15	ND	ND	ND	ND	4	7	44
TxCon 16	ND	ND	ND	ND	5	6	96
TxCon 17	ND	ND	ND	ND	5	6	22
TxCon 18	ND	ND	ND	ND	4	5	48
TxCon 19	ND	ND	ND	ND	7	8	71
TxCon 20	ND	ND	ND	ND	6	12	57
TxCon 21	ND	ND	ND	ND	5	10	34
TxCon 22	ND	ND	ND	ND	4	7	23
TxPh-1 #1	23	0	551	729	0	0	0
TxPh-1 #2	3	0	517	702	0	0	0
TxPh-1 #3	29	0	541	704	0	0	0
TxPh-1 #4	0	0	502	722	0	0	0
TxPh-1 #5	36	0	488	689	0	0	0
TxPh-1 #6	7	0	583	703	0	0	0
TxPh-1 #7	42	0	511	748	0	0	0
TxPh-1 #8	32	0	557	716	0	0	0
TxPh-1 #9	14	0	531	678	0	0	0
TxPh-1 #10	18	0	502	719	0	0	0
TxPh-1 #11	28	0	562	764	0	0	0
TxPh-1 #12	36	0	523	750	0	0	0
TxPh-1 #13	28	0	590	721	0	0	0
TxPh-1 #14	51	0	589	748	0	0	0
TxPh-2 #1	1,819	0	602	841	1	0	4
TxPh-2 #2	1,545	0	587	785	0	1	2
TxPh-2 #3	2,254	0	599	794	2	2	6
TxPh-2 #4	1,324	0	610	765	1	0	0
TxPh-2 #5	1,245	0	547	769	1	0	2
TxPh-2 #6	1,873	0	541	787	1	1	1
TxPh-2 #7	1,956	0	622	712	2	2	2
TxPh-2 #8	1,138	0	516	758	0	1	1
TxPh-2 #9	1,596	0	561	794	0	1	1

ND, not determined.