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Groove 3 and tetramerization are linked. (A) Characterization of the interaction between IAA12 EAR motif (FAM labeled) and TPL proteins impaired in tetramerization by fluorescence anisotropy assay. EC50 shown below the graph (B) HTRF competition assays performed on an initial GST-IAA12/MBP-AtTPL202 complex by adding increasing amounts of His-tagged AtTPL202 wt and mutant proteins. IC50 shown below the graph. a.u., arbitrary units.
