Fig. 6.

Importance of TPL G3 groove and tetramerization in auxin signaling. In planta repression assay in Col-0 protoplasts containing the integrated DR5::VENUS reporter gene. A loss of repression activity is observable in TPL mutants impaired in their interaction with repressors, their tetramerization, or carrying the tpl-1 mutation. DR5 activity is reported (n = 200 protoplasts). Error bars correspond to the 95% confidence interval. Two variables with different letters are significantly different (corrected P values < 0.05; two-sided Kruskal-Wallis test). a.u., arbitrary units.