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. 2017 Jul 10;114(30):8023–8028. doi: 10.1073/pnas.1703240114

Table S1.

Determined binding constants for the C2 domains of otoferlin with Loop1.3 or t-SNARE

Free calcium concentration C2A C2B C2C C2D C2E C2F
Loop1.3 (μM) SNAREs (μM) Loop1.3 (μM) SNAREs (μM) Loop1.3 (μM) SNAREs (μM) Loop1.3 (μM) SNAREs (μM) Loop1.3 (μM) SNAREs (μM) Loop1.3 (μM) SNAREs (μM)
0.1 μM Ca2+ 0.31 ± 0.03 0.56 ± 0.06 0.07 ± 0.01 1.19 ± 0.01 1.04 ± 0.04 0.03 ± 0.02 1.17 ± 0.01
1 μM Ca2+ 0.28 ± 0.02 0.57 ± 0.07 0.35 ± 0.04 1.00 ± 0.01 1.24 ± 0.01 0.12 ± 0.02 1.09 ± 0.01 1.79 ± 0.03
15 μM Ca2+ 0.44 ± 0.03 0.46 ± 0.01 0.80 ± 0.01 0.09 ± 0.02 0.66 ± 0.01 0.50 ± 0.08 0.49 ± 0.09 0.75 ± 0.01 0.83 ± 0.01
30 μM Ca2+ 0.31 ± 0.04 0.46 ± 0.02 1.51 ± 0.03 0.07 ± 0.01 0.10 ± 0.01 0.88 ± 0.02 0.30 ± 0.07 0.51 ± 0.01 0.05 ± 0.02
50 μM Ca2+ 0.38 ± 0.04 0.59 ± 0.02 0.29 ± 0.03 0.07 ± 0.01 0.15 ± 0.05 0.90 ± 0.08 0.09 ± 0.01 0.74 ± 0.05 0.04 ± 0.02