Table S1.
Strains used in this study
| Strain | Genotype | Construction/description | Source |
| AH109 | MATa, trp1-901, leu2-3, 112, ura3-52, his3-200, gal4Δ, gal80Δ, LYS2::GAL1UASGAL1TATA- HIS3, GAL2UAS-GAL2TATA-ADE2, URA3::MEL1UAS-MEL1TATA-lacZ | Host strain for yeast-two hybrid analysis | Clontech |
| TOP10 | F– mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara leu) 7697 galU galK rpsL (StrR) endA1 nupG | Cloning | Invitrogen |
| ET12567/pUZ8002 | F– dam13::Tn9 dcm6 hsdM hsdR recF143::Tn10 galK2 galT22 ara-14 lacY1 xyl-5 leuB6 thi-1 tonA31 rpsL hisG4 tsx-78 mtl-1 glnV44 | ET12567 containing helper plasmid pUZ8002 | 53 |
| BW25113/pIJ790 | Δ(araD-araB)567 ΔlacZ4787(::rrnB-4) lacIp-4000(lacIQ), λ−rpoS369(Am) rph-1 Δ(rhaD-rhaB)568 hsdR514 | BW25113 containing λ RED recombination plasmid | 54 |
| BTH101 | F– cya-99 araD139 galE15 galK16 rpsL1 (Strr) hsdR2 mcrA1 mcrB1 | Host strain for bacterial two-hybrid analysis | 55 |
| Streptomyces strains | |||
| WT | S. venezuelae ATCC 10712 | Laboratory strain | 52 |
| SV8 | ΔwhiH::apr | M. Bibb, unpublished | |
| ΔftsZ | ΔftsZ::apr | J. McCormick, unpublished | |
| LUV015 | attBϕC31::PftsZ-ftsZ-ypet | pKF351 integrated at the ΦC31 attachment site of S. venezuelae WT | This work |
| LUV001 | ΔdynAB::apr | Chromosomal dynAB locus was replaced with apra-oriT from pIJ773 by ‟Redirect” PCR targeting using oligo KF843 and KF844 | This work |
| SS2 | ΔdynB::apr | Chromosomal dynB locus was replaced with apra-oriT from pIJ773 by ‟Redirect” PCR targeting using oligo 8 and 9 | This work |
| SS4 | attBϕBT1::pIJ10257 | pIJ10257 integrated at the ΦBT1 attachment site of S. venezuelae WT | This work |
| SS10 | ΔdynAB::apr attBϕBT1::pIJ10257 | pIJ10257 integrated at the ΦBT1 attachment site of LUV001 | This work |
| SS11 | ΔdynAB::apr attBϕBT1::PermE*-ftsZ | pSS3 integrated at the ΦBT1 attachment site of LUV001 | This work |
| SS12 | attBϕBT1::PftsZ-ftsZ-ypet | pSS5 integrated at the ΦBT1 attachment site of S. venezuelae WT | This work |
| SS14 | ΔdynAB::apr attBϕBT1::PftsZ-ftsZ-ypet | pSS5 integrated at the ΦBT1 attachment site of LUV001 | This work |
| SS23 | ΔdynAB::apr attBϕBT1::Pdyn-dynAB | pKF337 integrated at the ΦBT1 attachment site of LUV001 | This work |
| SS39 | attBϕC31::PftsZ-ftsZ-mcherry | pSS61 integrated at the ΦC31 attachment site of S. venezuelae WT | This work |
| SS89 | ΔdynB::apr attBϕBT1::PermE*-dynB-mcherry | pSS87 integrated at the ΦBT1 attachment site of SS2 | This work |
| SS90 | ΔdynAB::apr attBϕBT1::Pdyn-dynA-dynB-ypet | pSS89 integrated at the ΦBT1 attachment site of LUV001 | This work |
| SS93 | attBϕBT1::PermE*-dynA-3xFLAG | pSS91 integrated at the ΦBT1 attachment site of LUV001 | This work |
| SS95 | ΔdynAB::apr attBϕBT1::PermE*-dynB-mcherry | pSS87 integrated at the ΦBT1 attachment site of LUV001 | This work |
| SS140 | ΔdynAB::apr attBϕBT1::PermE*-dynA-dynB-3xFLAG | pSS130 integrated at the ΦBT1 attachment site of LUV001 | This work |
| SS142 | ΔdynAB::apr attBϕBT1::PermE*-dynA-dynB-ypet | pSS134 integrated at the ΦBT1 attachment site of LUV001 | This work |
| SS164 | ΔdynAB::apr attB ϕBT1::PermE*-dynA-dynB[K129A]-ypet | pSS141 integrated at the ΦBT1 attachment site of LUV001 | This work |
| SS165 | ΔdynAB::apr attB ϕBT1::PermE*-dynA[K74A]-dynB[K129A]-ypet | pSS142 integrated at the ΦBT1 attachment site of LUV001 | This work |
| SS190 | ΔdynB::apr attBϕBT1::Pdyn-mcherry-dynA | pSS188 integrated at the ΦBT1 attachment site of SS2 | This work |
| SS196 | attBϕC31::PftsZ-ftsZ-ypet | pSS180 integrated at the ΦBT1 attachment site of LUV015 | This work |
| attBϕBT1::Pdyn-dynA-dynB-mcherry | |||
| SS205 | attBϕBT1::Pdyn-mcherry-dynA-dynB-ypet | pSS192 integrated at the ΦBT1 attachment site of S. venezuelae WT | This work |
| SS206 | attBϕC31::PftsZ-ftsZ-mturquoise2 attBϕBT1::Pdyn-mcherry-dynA-dynB-ypet | pSS192 integrated at the ΦBT1 attachment site of SS39 | This work |
| SS208 | attBϕC31::PftsZ-ftsZ-ypet attBϕBT1::PsepF-sepf-mcherry | pSS196 integrated at the ΦBT1 attachment site of LUV015 | This work |
| SS211 | ΔdynAB::apr attB ϕBT1::PermE*-dynA[K74A]-dynB-ypet | pSS208 integrated at the ΦBT1 attachment site of LUV001 | This work |
| SS213 | attBϕC31::PftsZ-ftsZ-ypet attBϕBT1::PsepF2-mcherry-sepf2 | pSS234 integrated at the ΦBT1 attachment site of LUV015 | This work |
| SS224 | attB ϕBT1::PermE*–ypet-dynA[K74A]-dynB[K129A] | pSS317 integrated at the ΦBT1 attachment site of WT S. venzuelae | This work |
| SS238 | ΔftsZ::apr attBϕBT1::PermE*-dynA-dynB-ypet | pSS134 integrated at the ΦBT1 attachment site of S. venezuelae ΔftsZ::apr | This work |
| SS242 | ΔdynAB::apr attB ϕBT1::PermE*-ypet-dynA-dynB | pSS310 integrated at the ΦBT1 attachment site of LUV001 | This work |
| SS244 | ΔdynAB::apr attB ϕBT1::PermE*-ypet-dynA-dynB[K129A] | pSS311 integrated at the ΦBT1 attachment site of LUV001 | This work |
| SS248 | ΔdynAB::apr attB ϕBT1::PermE*–ypet-dynA[K74A]-dynB | pSS316 integrated at the ΦBT1 attachment site of LUV001 | This work |
| SS250 | ΔdynAB::apr attB ϕBT1::PermE*–ypet-dynA[K74A]-dynB[K129A] | pSS317 integrated at the ΦBT1 attachment site of LUV001 | This work |
| SS255 | ΔdynA | pSS107 integrated at dynA locus of S. venezuelae WT via double homologous recombination | This work |
| SS262 | attBϕBT1::PermE*-dynA-dynBΔTM-ypet | pSS353 integrated at the ΦBT1 attachment site of S. venezuelae WT | This work |
| SS263 | ΔdynAB::apr attBϕBT1::PermE*-dynA-dynBΔTM-ypet | pSS353 integrated at the ΦBT1 attachment site of LUV001 | This work |
| SS266 | ΔdynAB::apr attBϕBT1::Pdyn-dynA-mcherry | pSS12 integrated at the ΦBT1 attachment site of LUV001 | This work |
| SS270 | ΔdynA attBϕBT1::PermE*-dynB-mcherry | pSS87 integrated at the ΦBT1 attachment site of SS255 | This work |