Fig. S3.

HEK293T cells were grown and treated as in Fig. 1A. (A) Cells were then lysed and subjected to SDS/PAGE followed by Western blot analysis against indicated antibodies. (B) Rate of turnover of [¹⁴C]L-valine–labeled long-lived proteins measured in HEK293T cells as in Fig. 1B. Results are expressed as fold increase of cellular protein degraded in 4 h, relative to cells in control medium. Data represent average of three independent experiments with SEM. (C) ACAT activity was measured in HEK293T cells. Results are represented as fold increases of CE formation, relative to cells in control medium. Data are averages of three independent experiments with SEM. **P < 0.005.