Fig. 4.

The role of ROS in mitochondrial calcium buffering capacity and membrane potential. (A) Ca²⁺ and TMRM monitoring of WT BAT mitochondria respiring on succinate/rotenone. (B) Ca²⁺ and TMRM monitoring of UCP1-KO BAT mitochondria respiring on succinate/rotenone. (C) Ca²⁺ and TMRM monitoring of UCP-KO BAT mitochondria respiring on succinate in the presence of 100 nM MitoQ. (D) Ca²⁺ and TMRM monitoring of WT BAT mitochondria respiring on G3P. (E) Ca²⁺ and TMRM monitoring of UCP1-KO BAT mitochondria respiring on G3P. Images shown are representative of at least three independent mitochondrial preparations for each substrate used. Arrows indicate the addition of mitochondria (red), GDP (blue), calcium (green), or MitoQ (yellow).