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. 2017 Jul 10;114(30):E6202–E6211. doi: 10.1073/pnas.1705491114

Table S2.

Primers used for quantitative real-time PCR

Gene Mus Primer Sequence, 5′-3′ Product size, bp Annealing T, °C
IL-1β F TGCCACCTTTTGACAGTGATG 138 60
R TGATGTGCTGCTGCGAGATT
IL-6 F AGCCAGAGTCCTTCAGAGAGAT 108 60
R AGGAGAGCATTGGAAATTGGGG
IL-10 F GGTGAGAAGCTGAAGACCCTC 134 60
R GCCTTGTAGACACCTTGGTCTT
IL-12a F CGGTCCAGCATGTGTCAATC 119 60
R GACTGGCTAAGACACCTGGC
IL-15 F GGAGCTGGAGGAGAAAACCTT 103 60
R AACACGGAAGAGGCTCGCAT
IL-18 F TCTTGGCCCAGGAACAATGG 142 60
R ACAGTGAAGTCGGCCAAAGT
CCL3 F CATATGGAGCTGACACCCCG 100 60
R GAGCAAAGGCTGCTGGTTTC
CCL2 F CTGCTGTTCACAGTTGCCG 200 60
R GCACAGACCTCTCTCTTGAGC
CCL4 F TGTGCAAACCTAACCCCGAG 106 60
R CCATTGGTGCTGAGAACCCT
CCL5 F GTGCTCCAATCTTGCAGTCG 200 60
R GGATTACTGAGTGGCATCCCC
CCL6 F CTTGTGGCTGTCCTTGGGTC 145 60
R TACATGGGATCTGTGTGGCA
CCL7 F CTTCTGTGCCTGCTGCTCATA 194 60
R TCCATGCCCTTCTTTGTCTTGA
CCL9/10 F TGGGTCTGCCCACTAAGAAGA 154 60
R TCAATTTCAAGCCCTTGCTGTG
CXCL2 F AGGGCGGTCAAAAAGTTTGC 184 60
R CAGGTACGATCCAGGCTTCC
CXCL4 F CAGTGGCACCCTCTTGACAT 135 60
R ATCGCTTTCTTCGGGACCAG
CXCL7 F CCTGGCGTCAAGAGAATCGT 101 60
R CAAACCCTCAACCCTTCCTGA
CXCL10 F AAGCTATGTGGAGGTGCGAC 183 60
R AACCCCTTGGGAAGATGGTG
CXCL11 F GGAAGGTCACAGCCATAGCC 174 60
R CAACTTTGTCGCAGCCGTTA
CXCL16 F TCGCAAAACATCAGTCAGGGT 137 60
R AAGAACGCAAGAGACAAGGGT
TNF-α F CGGGCAGGTCTACTTTGGAG 166 60
R ACCCTGAGCCATAATCCCCT
iNOS F TCCTGGACATTACGACCCCT 110 60
R CTCTGAGGGCTGACACAAGG
IFN-γ F GCAACAGCAAGGCGAAAAAG 139 60
R CGCTTCCTGAGGCTGGATTC
GM-CSF F CTGCTTTTGTGCCTGCGTAA 140 60
R TCGTTTGTCTTCCGCTGTCC
RAE-1 F AGTGACCAAGCGCCATCATT 137 60
R AGGGAAGGTCTGCTGAGGTA
MULT-1 F TGAGCTCATGTTGCACTGGA 161 60
R AGGACTGCAGGCCAATCTTT
HPRT-1 F TGCTGACCTGCTGGATTACA 120 60
R TTTATGTCCCCCGTTGACTGA
ChAT F AGGGCAGCCTCTCTGTATGA 241 60
R ATCCTCGTTGGACGCCATTT