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. 2017 Jul 10;114(30):8071–8076. doi: 10.1073/pnas.1706908114

Fig. S3.

Fig. S3.

Gene-expression profile. We generated ts-46 (A) and ts-101 (B) KO stable clones from HEK-293 cells by using CRISPR technology. RNA from two ts-46 KO clones, two ts-101 KO clones, and two samples transfected with the empty pCAS9 vector were used for Affymetrix gene-expression profiles. By setting a linear 1.5-fold change, we found 270 genes differentially expressed in the ts-46 clones and 216 genes differentially expressed in the ts-101 clones compared with the WT. Some examples of up-regulated genes in ts-46 and ts-101 clones are MAP3K19, miR-222, and MED28. As expected, mir-4521 is strongly down-regulated in ts-101 clones. Among down-regulated genes we found FILIP1, OCLN, and HIST1H3-A, -B, -C, -D, -E, -F, -G, -H, -I, -J, supporting our hypothesis that tsRNAs can interfere with chromatin structure. All values are in log2 scale; red depicts overexpression, and blue represents underexpression.