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M7Vs are distinct from well-characterized intracellular vesicular compartments. (A) Immunocytochemistry of VSVG-GFP and TRPM7-HA vesicles in HEK293 cells. (B) Absence of colocalization of TRPM7-HA and markers of the ER (Calnexin), EDEMosomes (Edem1), the tyrosinase ER–Golgi intermediate compartment, peroxisomes (RFP-PTS and Pex19), lysosomes (Lamp1), melanosomes (Tyrosinase or Tyros), or clathrin-coated endosomes. See Table S1 for additional description of markers.
