Table S1.
Probes used to label various compartments
| Reagent | Target protein | Organelle | Related figure(s) |
| Markers/antibodies | |||
| Anti-calnexin | Calnexin | ER | 2B |
| Anti-ERGIC-53 | ER-Golgi intermediate compartment 53 kDa protein | ER–Golgi intermediate compartment | 2B |
| Anti-Lamp1 | Lysosomal associated membrane protein 1 | Lysosome | 2B |
| Anti-clathrin | Clathrin-coated endosomes | Endosomes | 2B |
| Anti-Pex19 | Peroxisomal biogenesis factor 19 | Immature peroxisomes | 2B |
| Anti–β-actin | β-Actin | Cytoskeleton, subplasma membrane cytoskeleton | 1B |
| Anti-PDI | Protein disulfide isomerase | ER | 3 A and D |
| Anti–cyt-C | Cytochrome C | Mitochondria | 3D |
| VSVG-GFP | Vesicular stomatitis virus G glycoprotein | Transport vesicles | 2A |
| D1ER (used here as a marker, not Ca2+ sensor) | CFP/Citrine FRET pair coupled with M13/calmodulin | ER | 2B |
| Cell Light Peroxisomes-RFP BacMam (RFP-PTS) | RFP containing peroxisome targeting sequence, PTS (Ser-Lys-Leu) | Peroxisomes | 2B |
| GFP-Tyrosinase | Tyrosinase | Melanosomes | 2B |
| VIP36 | Vesicular integral membrane 36 | Post-ER vesicles | 5A |
| Edem1 | ER degradation-enhancing α-mannosidase–like protein 1 | EDEMosomes | 2B |
| GFP-TRPM7 | TRPM7 | M7V | 1E and Movie S1 |
| TRPM7-HA | TRPM7 | M7V | 1D |
| Sensors | |||
| Phluorin-TRPM7 | TRPM7 (pH sensor in S1–S2 linker) | M7V | 1D |
| GCaMP6s | NA (GFP-based Ca2+ sensor) | Cytosol/nucleus | S4 |
| R-GECO1 | NA (RFP-based Ca2+ sensor) | Cytosol/nucleus | S4 |
| RCEPIAer | NA (RFP-based low-affinity Ca2+ sensor) | ER | S4 |
| GCaMP6S-TRPM7 | TRPM7 (Ca2+ sensor in S1-S2 linker) | M7V | S4 and S2 |
| ZnGreen1-TRPM7 | TRPM7 (Zn2+ sensor in S1-S2 linker) | M7V | 5 I–L and S5 C, D, and F |
| ZnGreen1-TRPM7 NT. | TRPM7 N-terminal | Cytosol | 5 K and L and S5 D and E |
| eCALWY4 | CFP/citrine FRET pair coupled with ATOX/WD4 Zn2+ binding domains | Cytosol/nucleus | 5G and 6 D–H |
| VIP36-eCALWY4 | VIP36 | M7V | 5 B, D, E, G, and H, 7 A–C and S5 A and B |
| Sec61β-eCALWY4 | Sec61β | ER-lumen | 5 C and F |
| roGFP2 | NA (ratiometric redox sensor based on cysteines inserted in GFP) | Cytosol/nucleus | 6 B and C |
| roGFP2-TRPM7 | M7V (redox sensor in S1-S2 linker) | M7V | 6 B and C |
| Indicators | |||
| Fluo4-AM | NA | Multiple | S4 |
| MagFluo4-AM | NA | Multiple | S4 |
| Fluozin-3-AM | NA | Multiple | 3, 6D, and 7 |
Sources of reagents not made in this study are described in Methods.