Table S1.
Bacterial strains and plasmids
| Strain | Description/use | Source |
| E. coli | ||
| CFT073 | Bacteremia isolate, wild-type (O6:K2:H1) | 28 |
| TOP10 | pWQ601, general cloning strain | Invitrogen |
| CFT073 lnt | CFT073 Δlnt::kan | This study |
| Plasmids | ||
| pKD4 | Kanamycin resistance (KanR) cassette flanked by FRT (FLP recognition target) sites, oriRγ | 42 |
| pKD46 | Expresses the phage λ Red recombinase, AmpR, temperature sensitive, oriRγ | 42 |
| pCP20 | Thermal induction of FLP recombinase expression, AmpR, temperature sensitive | 43 |
| pLMG18tet | IPTG-inducible lacI promoter, low copy plasmid, TetR, p15a origin | This study |
| pET52 | T7lac promoter, AmpR, high copy plasmid, f1 origin | Novagen |