Table S2.
Primers used in this study
| Primer | Sequence, 5′ to 3′ |
| Generation of CFT073 lnt | |
| CFT073 lnt.F | CACCCCAGCCGAAGCTGGATGAATAAAACCGAAACTGGATAGATAACTACGTGTAGGCTGGAGCTGCTTC |
| CFT073 lnt.R | CCACAACTCAATGAGAAAGTATGTTTTGTTCATGCCGGATACATCAGGCACACATATGAATATCCTCCTTAGTTCCTATTC |
| Site-directed mutagenesis for pLMG18tet complementation | |
| pLMG18tet.Lnt-his.F | GGAATTCGAGCTCGGTACCATGGCTTTTGCCTCATTAATTGAAC |
| pLMG18tet.Lnt-his.R | TGCAGGTCGACTCTAGATCAATGATGATGATGATGATGATGTTTACGTGCTCGCAGACTC |
| pLMG18tet.LntC23A-his.F | TTCGGTGCCTGCGGAACGCTG |
| pLMG18tet.LntC23A-his.R | CAGCGTTCCGCAGGCACCGAA |
| pLMG18tet.LntC62A-his.F | ATTGGCTTTGCCTGGGGATTTG |
| pLMG18tet.LntC62A-his.R | CAAATCCCCAGGCAAAGCCAAT |
| pLMG18tet.LntI74A-his.F | GGTATTAACGCGGTCTATGTC |
| pLMG18tet.LntI74A-his.R | GACATAGACCGCGTTAATACC |
| pLMG18tet.LntI79A-his.F | CTATGTCAGCGCCGCGACCTTTG |
| pLMG18tet.LntI79A-his.R | CAAAGGTCGCGGCGCTGACATAG |
| pLMG18tet.LntF82A-his.F | CATCGCGACCGCTGGCGGAATG |
| pLMG18tet.LntF82A-his.R | CATTCCGCCAGCGGTCGCGATG |
| pLMG18tet.LntF82A-his.F | CATCGCGACCGCTGGCGGAATG |
| pLMG18tet.LntF82A-his.R | CATTCCGCCAGCGGTCGCGATG |
| pLMG18tet.LntF146A-his.F | CTAACAGGCGCCCCGTGGTTAC |
| pLMG18tet.LntF146A-his.R | GTAACCACGGGGCGCCTGTTAG |
| pLMG18tet.LntW148A-his.F | CAGGCTTCCCGGCGTTACAGTTC |
| pLMG18tet.LntW148A-his.R | GAACTGTAACGCCGGGAAGCCTG |
| pLMG18tet.LntK220A-his.F | CAACCGGAAGCAACTATTCAG |
| pLMG18tet.LntK220A-his.R | CTGAATAGTTGCTTCCGGTTG |
| pLMG18tet.LntQ233A-his.F | GATATTCCGGCATCGCTGAAATG |
| pLMG18tet.LntQ233A-his.R | CATTTCAGCGATGCCGGAATATC |
| pLMG18tet.LntK236A-his.F | CGCAATCGCTGGCATGGGACGG |
| pLMG18tet.LntK236A-his.R | CCGTCCCATGCCAGCGATTGCG |
| pLMG18tet.LntW237A-his.F | CCGCAATCGCTGAAAGCGGACGGAGACCAGC |
| pLMG18tet.LntW237A-his.R | GCTGGTCTCCGTCCGCTTTCAGCGATTGCGG |
| pLMG18tet.LntW237E-his.F | CGCTGAAAGAGGACGGAGACC |
| pLMG18tet.LntW237E-his.R | GGTCTCCGTCCTCTTTCAGCG |
| pLMG18tet.LntW267A-his.F | CGTTGATTATCTGGCCGGCGTCGGCGATAACCGATC |
| pLMG18tet.LntW267A-his.R | GATCGGTTATCGCCGACGCCGGCCAGATAATCAACG |
| pLMG18tet.LntE267D-his.F | CTGGCCGGACTCGGCGAT |
| pLMG18tet.LntE267D-his.R | ATCGCCGAGTCCGGCCAG |
| pLMG18tet.LntN314A-his.F | CGATACCTACGCCACCATCATC |
| pLMG18tet.LntN314A-his.R | GATGATGGTGGGCTAGGTATCG |
| pLMG18tet.LntW335A-his.F | GCCGATCGCTATAACGCAAACCATCTGGTGCCG |
| pLMG18tet.LntW335A-his.R | CGGCACCAGATGGTTTGCGTTATAGCGATCGGC |
| pLMG18tet.LntF314A-his.F | CTGGTGCCGGCTGGCGAG |
| pLMG18tet.LntF341A-his.R | CTCGCCAGCCGGCACCAG |
| pLMG18tet.LntW343A-his.F | CTGGTGCCGTTTGGCGCGTTTGTCCCACTGG |
| pLMG18tet.LntW343A-his.R | CCAGTGGGACAAACGCGCCAAACGGCACCAG |
| pLMG18tet.LntE343D-his.F | GCCGTTTGGCGACTTTGTCCC |
| pLMG18tet.LntE343D-his.R | GGGACAAAGTCGCCAAACGGC |
| pLMG18tet.LntF365A-his.F | ATGTCTTCAGCCAGCCGCGG |
| pLMG18tet.LntF365A-his.R | CCGCGGCTGGCTGAAGACAT |
| pLMG18tet.LntW387A-his.F | CTTACTGCGGCCATTGCCTACGAAATCATTCTCGGCG |
| pLMG18tet.LntW387A-his.R | CGCCGAGAATGATTTCGTAGGCAATGGCCGCAGTAAG |
| pLMG18tet.LntW387S-his.F | CTTACTGCGGCCATTTCTTACGAAATCATTCTCGGCG |
| pLMG18tet.LntW387S-his.R | CGCCGAGAATGATTTCGTAAGAAATGGCCGCAGTAAG |
| pLMG18tet.LntY388A-his.F | CTTACTGCGGCCATTTGCGCCGAAATCATTCTCGGCG |
| pLMG18tet.LntY388A-his.R | CGCCGAGAATGATTTCGGCGCAAATGGCCGCAGTAAG |
| pLMG18tet.LntY388F-his.F | CCATTTGCTTCGAAATCATTCT |
| pLMG18tet.LntY388F-his.R | AGAATGATTTCGAAGCAAATGG |
| pLMG18tet.LntY389A-his.F | CTGCGGCCATTTGCTACGCAATCATTCTCGGCGAGC |
| pLMG18tet.LntY389A-his.R | GCTCGCCGAGAATGATTGCGTAGCAAATGGCCGCAG |
| pLMG18tet.LntW415A-his.F | AACGATGCGGCGTTTGGTAAG |
| pLMG18tet.LntW415A-his.R | CTTACCAAACGCCGCATCGTT |
| pLMG18tet.LntF416A-his.F | CGATGCGTGGGCTGGTAAGTC |
| pLMG18tet.LntF416A-his.R | GACTTACCAGCCCACGCATCG |
| Site-directed mutagenesis for recombinant Lnt protein expression | |
| pET.LntW237A-his.F | GATATTCCGCAGTCTCTGAAAGCGACGAAGGCCAGCTGCTGAACACGC |
| pET.LntW237A-his.R | CGTGTTCAGCAGCTGGCCTTCGTCGCTTTCAGAGACTGCGGAATATC |
| pET.LntW267A-his.F | GCAGCCTGATTATCTGGCCGGCGGCGCTATTACCGATCTGG |
| pET.LntW267A-his.R | CCAGATCGGTAATAGCGCCGCCGGCCAGATAATCAGGCTGC |
| pET.LntW335A-his.F | GCGCGGATCGCTATAACGCGAATCATCTGGTCCCGTTTGGTG |
| pET.LntW335A-his.R | CACCAAACGGGACCAGATGATTCGCGTTATAGCGATCCGCGC |
| pET.LntW343A-his.F | TCATCTGGTCCCGTTTGGTGCGTTTGTGCCGCTGGAATCTATTC |
| pET.LntW343A-his.R | GAATAGATTCCAGCGGCACAAACGCACCAAACGGGACCAGATGA |
| pET.LntW387A-his.F | TTGAACTGACCGCGGCGATTGCGTATGAAATTATCCTGGGCGAAC |
| pET.LntW387A-his.R | GTTCGCCCAGGATAATTTCATACGCAATCGCCGCGGTCAGTTCAA |
| pET.LntW387S-his.F | TTGAACTGACCGCGGCGATTTCGTATGAAATTATCCTGGGCGAAC |
| pET.LntW387S-his.R | GTTCGCCCAGGATAATTTCATACGAAATCGCCGCGGTCAGTTCAA |
| pET.LntY388A-his.F | GAACTGACCGCGGCGATTTGCGCGGAAATTATCCTGGGCGAACAAGTG |
| pET.LntY388A-his.R | CACTTGTTCGCCCAGGATAATTTCCGCGCAAATCGCCGCGGTCAGTTC |
| pET.LntY389A-his.F | CTGACCGCGGCGATTTGCTATGCGATTATCCTGGGCGAACAAGTGCG |
| pET.LntY389A-his.R | CGCACTTGTTCGCCCAGGATAATCGCATAGCAAATCGCCGCGGTCAG |