Skip to main content
. Author manuscript; available in PMC: 2017 Aug 4.
Published in final edited form as: Curr Mol Med. 2016;16(3):252–265. doi: 10.2174/1566524016666160225153307

Figure 4. Expression of p27 is decreased in obese Tsc2-deficient rats and reversed by caloric restriction.

Figure 4

mTORC1 activation (phosphorylation at (S235/236) of S6 ribosomal protein) was present in 6% of normal appearing glands (A) and in 100% of endometrial hyperplastic (EH) lesions (C). p27 nuclear localization was intact in normal endometrial epithelium that do not have activated mTORC1 signaling (B) and was decreased or absent in endometrial hyperplasia (EH)(D). Eker rats treated with genestein neonatally developed EH exhibiting mTORC1 activation in ad libitum fed rats (E) and in calorie restriction (CR) rats (G). ad libitum fed rats had decreased or absent nuclear p27 in EH lesions (F). Caloric restriction restores and increases the nuclear expression of p27 in EH lesions (H). Arrows indicate glandular epithelial cells with mTORC1 activation that was inversely correlated with nuclear p27 expression. Original magnification, ×200.