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. 2017 Jul 27;101(2):283–290. doi: 10.1016/j.ajhg.2017.07.001

Figure 5.

Figure 5

Rescue of Protein Lipoylation in Affected Individuals with LIPT2 Deficiency

Fibroblasts from affected individuals were transfected with wild-type LIPT2. Immunostaining analysis of protein-bound lipoic acid showed increase of protein lipoylation after transfection. The following antibodies and reagents were used: rabbit anti-LIPT2 (Novus Biological, ImmunoFluorescence [IF]: 1:100), rabbit anti-lipoic acid (Abcam cat# ab58724, RRID: AB_880635; 1:1,000), and DAPI 300 nM in PBS (D1306, Molecular Probes).