Figure 1. Disruption of RNA binding proteins via targeted CRISPR/Cas9-mediated homologous recombination.
(A) Schematic of template-directed homologous recombination to disrupt a gene of interest and replace with a positive-selection cassette (NeoR = Neomycin resistance) and one of two tissue specific fluorescent marker genes labeling the pharynx or body wall musculature. (B) Double mutants are created by simple crosses on the fluorescent dissection microscope looking for presence of both fluorescent markers.