Fig. 4.

(A) Relative protein expressions of NADPH oxidase subunits, p47phox, rac-1, NOX2, and Na⁺/K⁺ ATPase in the membrane fraction from human coronary arterial smooth muscle cells 60 min after addition of L-, D-glucose (20 mmol/L) or D-glucose in combination with human serum albumin (BENESIS, 0.5 g/dL). *: P < 0.05 vs. L-glucose. Note that p47phox images split were taken from the same picture in the Supplementary Figure, where the full, non-adjusted images are available. (B) Immunofluorescent image analysis in the human coronary arterial smooth muscle cells 60 min after addition of L-, D-Glucose or D-Glucose in combination with human serum albumin (BENESIS, 0.5 g/dL) from five separate experiments. P47phox (green) was localized to the cytoplasmic region whereas it migrates to the cell periphery after incubation with D-glucose.