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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Mol Cancer Ther. 2017 May 31;16(8):1658–1668. doi: 10.1158/1535-7163.MCT-17-0114

Figure 4. CDC25C overexpression in KRAS mutant NSCLC cell lines leads to bypass of the ganetespib induced G2/M arrest and docetaxel resistance.

Figure 4

Representative cell cycle analysis at 48 hours after treatment with ganetespib in (A) H460 and (B) A549 cells carrying either empty vector W118Δ as control or the CDC25C. Bar graph represents a comparative display of % of G2/M cells corresponding to the flow cytometry data. CDC25C overexpression significantly decreased the % G2/M in response to 50 nM ganetespib as determined by performing unpaired t-test on the % G2/M to obtain the level of significance and denoted as **, p < 0.001(C) Representative MTS assay at 72 hours after treatment with docetaxel in control vs. CDC25C overexpressing KRAS mutant NSCLC cell lines. Unpaired t-test was performed on the IC50 values obtained from three independent biological repeats to determine the level of significance between control and experimental groups and denoted as **, p < 0.01 and ***, p < 0.001. See Supplementary Table S3 for further details.