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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Mol Cancer Ther. 2017 Apr 20;16(8):1680–1692. doi: 10.1158/1535-7163.MCT-16-0923

Figure 1. Variable GR expression and transcriptional activity in PC cells is dependent on AR function.

Figure 1

For A-C LAPC4 and CWR-22Rv1 cells were treated with vehicle (Veh), R1881 (R, 1nM) +/− dexamethasone (D, 100nM) for three days. A. Cell lysates were analyzed by Western blot for AR (full length (fl) and AR-V7 splice variant are indicated), GR, and β-actin. B. RNA was isolated and SGK1 and KLK3 mRNA levels were quantified by qRT-PCR. C. Supernatants were analyzed by ELISA to measure PSA (top panels) and cell lysates were analyzed by Western blot for SGK1, and β-actin (lower panels). D. Cells were treated as in A for 10 days and assessed for the total number of viable cells by trypan blue exclusion assay. Data is representative of 3 independent experiments. Error bars represent SEM. Statistical significance was determined by Student’s t-test, *p<0.05, **p<0.01.