Skip to main content
. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Mol Cancer Ther. 2017 Jun 1;16(8):1470–1486. doi: 10.1158/1535-7163.MCT-17-0134

Figure 6. HuR-TfNP treatment suppresses experimental lung metastasis.

Figure 6

Mice bearing A549-luc lung tumors were treated i.v. with C-TfNP or HuR-TfNP for a total of six treatments. Mice were imaged at regular intervals to monitor and measure tumor growth. A. Representative bioluminescence images showing inhibition of experimental lung metastasis in HuR-TfNP-treated mice compared to C-TfNP-treated mice. Bar graph represents the quantitation of bioluminescence intensity as Average Radiance [p/s/cm²/sr]. The gradual decrease in the bioluminescence signals in HuR-TfNP compared to C-TfNP groups suggests significant reduction in tumor growth over the period of treatment. B. Western blot image shows reduced HuR expression in HuR-TfNP-treated A549 lung tumors (upper panel). Sample # 1 and # 2 are representative samples for each treatment group analyzed at end of the study. Lung tumor nodules of a representative animal from each treatment group are also shown (middle panel). Animals were euthanized after six weeks; lungs were inflated with bovine solution and the number of extrapulmonary tumor nodules counted (lower panel). A significant reduction in the number of lung nodules were observed in HuR-TfNP-treated animals compared to control. C. Images represent the IHC staining of lung sections for HuR, CD31, and Ki67. Hematoxylin and eosin (H&E) stain showing the tissue pathology with dashed circle marking tumors in the lung. Bar graph represents the quantitation of IHC stains using. * p<0.05, ** p<0.001.