. 2017 Aug 4;7(5):276. doi: 10.1007/s13205-017-0905-4

Table 2.

Optimization of KA production through selection of incubation temperature after different incubation time

Incubation period (days)	Temperature (°C)	A. flavus HAk1			A. oryzae HAk2
Incubation period (days)	Temperature (°C)	Dry weight (g/100 ml) culture medium	KA (g/100 ml) culture medium	% Antioxidant	Dry weight (g/100 ml) culture medium	KA (g/100 ml) culture medium	% Antioxidant
5	20	0.9067 ± 0.03^ab	0.0466 ± 0.006^ab	39.36 ± 2.01	1.253 ± 0.06^ab	0.0397 ± 0.01^ab	34.12 ± 1.02
	30	1.2933 ± 0.19^ac	0.1489 ± 0.04^ac	49.2 ± 1.89	1.36 ± 0.04^ab	0.1071 ± 0.009^ad	53.6 ± 1.91
	40	1.1133 ± 0.04^ac	0.1132 ± 0.04^ab	42 ± 2.68	0.92 ± 0.01^ab	0.0682 ± 0.007^ac	51.68 ± 3.02
	50	0.00 ± 0.00^a	0.00 ± 0.00^a	0.00 ± 0	0.00 ± 0.00^a	0.00 ± 0.00^a	0.00 ± 0
7	20	1.0876 ± 0.1^b	0.2005 ± 0.01^bc	52.4 ± 3.42	0.933 ± 0.18^ab	0.0359 ± 0.01^ab	48 ± 2.00
	30	1.554 ± 0.05^bc	0.4557 ± 0.06^c	63.08 ± 3.21	1.366 ± 0.15^ac	0.1126 ± 0.03^ad	56.28 ± 2.22
	40	1.100 ± 0.17^bc	0.2722 ± 0.03^bc	53.96 ± 1.69	1.07 ± 0.03^ab	0.1019 ± 0.04^ac	39.16 ± 2.43
	50	0.00 ± 0.00^ab	0.00 ± 0.00^a	0.00 ± 0	0.00 ± 0.00^a	0.00 ± 0.00^a	0.00 ± 0
10	20	1.1467 ± 0.09^b	0.1563 ± 0.06^b	61.92 ± 2.74	1.046 ± 0.11^cb	0.0566 ± 0.01^b	51.48 ± 1.89
	30	1.160 ± 0.06^abc	0.2146 ± 0.07^abc	62.88 ± 2.35	1.54 ± 0.21^c	0.2275 ± 0.03^bd	62.48 ± 2.12
	40	1.30 ± 0.08^abc	0.1034 ± 0.01^b	48.88 ± 1.77	1.42 ± 0.04^cb	0.1513 ± 0.05^bc	51.4 ± 2.76
	50	0.00 ± 0.00^ab	0.00 ± 0.00^a	0.00 ± 0	0.00 ± 0.00^a	0.00 ± 0.00^a	0.00 ± 0
14	20	1.16 ± 0.17^b	0.0694 ± 0.03^ab	48.6 ± 2.09	1.186 ± 0.25^abc	0.0543 ± 0.01^ab	46.8 ± 1.42
	30	1.12 ± 0.02^bc	0.1403 ± 0.04^c	48.08 ± 2.99	1.44 ± 0.06^abc	0.1035 ± 0.05^ad	55.28 ± 3.2
	40	1.4467 ± 0.12^bc	0.0643 ± 0.01^ab	44.8 ± 0.89	1.246 ± 0.14^abc	0.0318 ± 0.04^ac	50.08 ± 2.06
	50	0.00 ± 0.00^ab	0.00 ± 0.00^a	0.00 ± 0	0.00 ± 0.00^a	0.00 ± 0.00^a	0.00 ± 0

Calculated mean is for triplicate measurements from two independent experiments ±SD; the values followed by different letters vary significantly at p ≤ 0.05 (Duncan test, p ≤ 0.05)