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. 2017 Aug 4;7:7284. doi: 10.1038/s41598-017-07246-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Splenocyte analysis in animals vaccinated with the adjuvanted and unadjuvanted Ad-ME.TRAP vaccine. Two weeks after immunisation of BALB/c mice (n = 6) with Ad-ME.TRAP (5 × 10⁹ vp/dose), alone or adjuvanted with Abisco^®-100 or CoVaccineHT, relative proportion of different cell subsets in the spleen were analysed by flow cytometry. Data are shown for CD4 and CD8 T cells (panels a and b). In parallel, production of IFNγ, TNFα and IL2 and degranulation (CD107a expression) by CD8⁺ splenocytes, following 4 h of in vitro stimulation with Pb9 peptide, was assessed by intracellular staining and flow cytometry (c–f). Representative FACS dot plot data are shown in the Supplementary Figure S2b. Ranked one-way ANOVA analysis (Kruskal-Wallis) with Dunn’s test for multiple comparisons was used to assess statistical significance between groups; *p < 0.05, **p < 0.01, ***p < 0.001. Data shown are representative of three independent experiments.