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. 2017 Aug 4;7:7284. doi: 10.1038/s41598-017-07246-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Memory profiles of CD8⁺ T cells in the peripheral blood, spleen and liver of animals vaccinated with unadjuvanted and adjuvanted Ad-ME.TRAP. BALB/c mice (n = 8) were immunized intramuscularly with Ad-ME.TRAP (5 × 10⁹ vp/dose), alone or adjuvanted with Abisco^®-100 or CoVaccineHT. Proportion of Pb9-specific CD8⁺ T cells in the peripheral blood, spleen and liver, and their memory profiles (T_E, T_EM and T_CM) were evaluated by flow cytometry using Pb9-tetramer and surface markers CD127 and CD62L. The three memory cell subsets were defined as: T_E = CD62L⁻CD127⁻, T_EM = CD62L⁻CD127⁺ and T_CM = CD62L⁺CD127⁺. Representative FACS data are shown in the Supplementary Figure S2c. Ranked one-way ANOVA analysis (Kruskal-Wallis) with Dunn’s test for multiple comparisons was used to assess statistical significance of the differences between groups; *p < 0.05, **p < 0.01, ***p < 0.001.