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. 2017 Aug 4;7:7349. doi: 10.1038/s41598-017-07461-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Photoreactions of DM-solubilized Drosophila Rh7. Difference spectra of DM-solubilized Rh7 regenerated with 11-cis-retinal (A) and 11-cis-3-hydroxyretinal (B) are shown. The black curve was calculated by subtracting the spectrum before irradiation from that after UV light irradiation. The red curve was calculated by subtracting the spectrum after UV light irradiation from that after subsequent yellow light (>500 nm) irradiation. Inset shows the superposition of difference spectra shown by red curves in (A) and (B). Spectra of samples regenerated with 11-cis-retinal (red curve) and 11-cis-3-hydroxyretinal (green curve) were normalized to be ~1.0 at the negative maximum.