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. 2017 Aug 4;7:7343. doi: 10.1038/s41598-017-07566-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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dG9a is critical for survival under starvation stress. (A) The results of a viability assay under starvation conditions using females of the wild-type (Canton S) (n = 153 from 8 independent experiments) and dG9a null mutant (dG9a^RG5) (n = 135 from 7 independent experiments) P < 0.0001 (B). The results of a viability assay under starvation conditions using males of the wild-type (n = 131 from 7 independent experiments) and dG9a null mutant (dG9a^RG5) (n = 138 from 7 independent experiments) P < 0.0001. (C) The results of a viability assay under heat stress using males of the wild-type (n = 80 from 4 independent experiments) and dG9a null mutant (dG9a^RG5) (n = 80 from 4 independent experiments) P = 0.41. (D) The results of a viability assay under oxidative stress using males of the wild-type (n = 100 from 5 independent experiments) and dG9a null mutant (dG9a^RG5) (n = 97 from 5 independent experiments) P = 0.80. (E) The results of a viability assay under starvation conditions using males of the FB (+; FB-GAL4; +) (n = 57 from 3 independent experiments) and FB > dG9a IR (w; UAS-dG9a IR/FB-GAL4; +) (n = 34 from 2 independent experiments) P < 0.0001. (F) The results of a viability assay under starvation conditions using males of the FB > dG9a IR (w; UAS-dG9a IR/FB-GAL4; +) (n = 59 from 3 independent experiments), males of the dG9a IR (w; UAS-dG9a IR/+; +) (n = 92 from 5 independent experiments) and males of Canton S (n = 60 from 3 independent experiments). dG9a IR and Canton S: P > 0.05. (A–F) Error bars represent standard errors (SE).