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. 2017 Aug 4;7:7343. doi: 10.1038/s41598-017-07566-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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dG9a is responsible for the induction of autophagy under starvation stress. (A) Immunostaining of wild-type and dG9a^RG5 mutant fat bodies under starvation conditions with an anti-Atg8a antibody. Strains: (a–d) Canton S (e) w; FB-GAL4/+; Atg8a^HMS01328/+ (f–i) dG9a^RG5. Starved hours: (a,f) 0 h (b,g) 6 h (c,h) 12 h (d,i) 24 h. Scale bars, 10 μm. (B) A western blot analysis of extracts from the starved wild-type and dG9a^RG5 mutant. Blots were probed with anti-Atg8a and anti-α-tubulin antibodies. Signal intensity normalized with that of α-tubulin is shown. The full-length image of the blot is shown in Fig. S6B. (C) Quantification of mRNA levels by a RT-qPCR analysis of Atg8a in the starved wild-type and dG9a^RG5 mutant. Results were normalized to α-tubulin and displayed as relative values to that of the 0-h starved wild-type n = 3. *P < 0.05. (D) The results of a viability assay under starvation conditions using the males of dG9a^RG5, FB > Atg8a (dG9a^RG5; FB-GAL4/Atg8a^{Scer/UAS.P/T.T:Avic/GFP-EGFP,T:Disc/RFP-mCherry}; +) (n = 39 from 2 independent experiments) and FB > Atg8a (+; FB-GAL4/Atg8a^{Scer/UAS.P/T.T:Avic/GFP-EGFP,T:Disc/RFP-mCherry}; +) (n = 38 from 2 independent experiments) strains P = 0.79. (C,D) Error bars represent SE.