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. 2017 Aug 4;7:7316. doi: 10.1038/s41598-017-06704-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fraction of P. brassicae egg survival under greenhouse conditions. (A) Impact of number of eggs per plant and egg distribution. Effect of HR expression on survival (mean fraction ± SE) of single P. brassicae eggs per plant and P. brassicae egg clusters of different size as stated on the X-axis (small: 10, medium: 50 and large: 90 eggs) laid on B. nigra plants. Survival rates of P. brassicae eggs on B. nigra plants expressing HR (light grey columns) or not (dark grey columns). Numbers in the columns represent the numbers of tested plants. (B) Impact of egg distribution. Effect of HR expression on survival (mean fraction ± SE) of 10 singly laid P. brassicae eggs and of a P. brassicae egg cluster (containing 10 eggs) on B. nigra plants under greenhouse conditions. Survival rates of eggs on B. nigra plants expressing HR (light grey columns) or not (dark grey columns). Numbers in the columns represent the numbers of tested plants. Significant differences are indicated using asterisks. *P < 0.05, ns: not significant, GLM.