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. 2017 Aug 4;7:7316. doi: 10.1038/s41598-017-06704-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Determination of HR severity induced by different numbers of P. brassicae eggs distributed singly or clustered in different clutch size on B. nigra plants. Mean fractions of plants expressing different degrees of HR severity are given. Each shade of grey represents a different HR severity, and the numbers above each column show the number of plants tested (also represented by the width of the bars). (A) Under greenhouse conditions with single P. brassicae eggs and P. brassicae egg clusters of different size (small: 10, medium: 50 and large: 90 eggs) in categories as stated on the X-axis. (B) Under field conditions with the following categories of egg clutch size: Small: ≥6 < 50 eggs, Medium: ≥50 < 100 eggs and Large: ≥100 eggs.