Skip to main content
. 2017 Aug 4;7:7262. doi: 10.1038/s41598-017-07724-5

Figure 3.

Figure 3

Endophilin B2 limits post-viral surfactant and lung cell depletion. (A,B) The levels of surfactant protein B in bronchoalveolar lavage (BAL) after infection were assessed in Western blot (A) by densitometry (B) and expressed relative to vehicle control. *p < 0.039 at t = 7 days and *p < 0.02 at t = 14 days for WT and B2−/− mice, respectively. Panel A shows representative Western blots from n = 2 independent experiments. (C–E) Change in wet lung weight (C), total BAL protein (D), and BAL albumin (E) after infection were measured by gravity, BCA, and ELISA, respectively. For WT mice, n = 5, 3, 6, and 5, and for B2−/− mice, n = 5, 6, 7, and 7 at t = 0, 3, 7, and 14 days after infection mice, respectively. (F) The number of live lung cells was assessed by flow cytometry. *p < 0.05 at t = 12 for B2−/− mice compared to WT. (n = 3 mice per group at t = 0, 3, and 7, and n = 12 at t = 12 from three independent determinations at 12 days after infection).