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. 2017 Aug 4;7:7292. doi: 10.1038/s41598-017-07402-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Axonal MTs and docked mitochondria translocate anterogradely during axonal elongation. (A) Still images of a chick DRG growth cone at the beginning and end of a time-lapse sequence in DIC, mitochondrial, MT fluorescent channels, and merged channels. Yellow arrow marks the T zone. (B) Kymograph showing motion. Red arrows indicate a mitochondrion and MT speckle undergoing fast transport. Scale and time bar as indicated. (C) Traces used to measure motion; arrow 10 min and bar 10 μm. (D) Input and output of LKMTA and KymoFlow algorithms for a subset of angles with color coded local velocities. (E) Input versus output shown as the average ± sd of velocity in units of p/f. (F) Average absolute error in degrees as a function of input angle. (G) Raw and sheared KymoFlow maps with color-coded velocities; arrow 10 min and bar 10 μm. (H) Velocity as a function of distance for the shown example. Position 0 corresponds with the growth cone T zone, where retrograde flow transitions into anterograde motion. (I) Velocity ± 95% CI as a function of distance for 31 neurons. (J) Regression analysis of docked mitochondrial and axonal MT velocity for 31 neurons.