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. 2017 Aug 4;7:7292. doi: 10.1038/s41598-017-07402-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phase-dense material and MTs move anterogradely during axonal elongation. (A) Phase contrast and MT FSM still images of a chick DRG neurite at the beginning and end of a time-lapse sequence. (B) Phase and MT kymographs showing anterograde flow; arrow = 5 min, bar = 10 μm. The red arrows are guides to illustrate motion. (C) Velocity ± 95% CI of phase-dense objects and MTs in 26 neurons. (D) Regression analysis of the motion of phase-dense objects and MTs.