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. 2017 Aug 4;7:7292. doi: 10.1038/s41598-017-07402-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The pattern of MT motion is conserved between chick and Aplysia. Still images at the beginning and end of time-lapse sequences in (A) Aplysia and (B) chick neurons. Kymographs of (C) Aplysia and (D) chick neurons with red arrows indicating anterograde motion of MTs in axons and retrograde flow of phase dense material in the growth cone P domain; arrows = 10 min, bars = 10 μm. (E) MT velocity as a function of distance from the T zone. (F) Regression analysis of axonal MT and growth cone velocity. (G) Regression analysis of growth cone and retrograde flow velocity.