Table 1.
(Wang and Brown)10 | (He et al.)8 | Our work | |
---|---|---|---|
Anterograde flux (MT/min) | 0.21 | 0.56 | 0.5 |
Retrograde flux (MT/min) | 0.03 | 0.3 | 0.38 |
Net flux (MT/min) | 0.18 | 0.26 | 0.12 |
Net flux (MT/h) | 10.6 | 15.6 | 7.2 |
Average MT length (μm) | 2.7 | 3.59 | 3.59 |
Transported MT length (μm/h) | 29 | 56 | 26 |
Ave. # of MT per cross section | 22 | 22 | 22 |
Net Axonal Growth (μm/h) | 1.3 | 2.5 | 1.2 |
Anterograde flux and retrograde flux refer to the number of MTs that move past a point along the axon over a given period of time in the respective directions. Net flux is determined by subtracting retrograde flux from anterograde flux. The transported length of MTs per hour is calculated by multiplying net flux by the average length of the transported MTs. Since that parameter was not measured in our study, we used the larger of the two values8 from the previous studies. Rochlin et al.16 reports a value of 22 MT/axonal cross section in sympathetic neurons, which have a similar morphology to sensory neurons. Net axonal growth is calculated by dividing transported MT length over time by the average number of MTs per axonal cross section.