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. 2017 Aug 4;7:7270. doi: 10.1038/s41598-017-07552-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Role of probe size in the interaction with mucus blankets. (a) Representative SEM image of the 40 µm colloidal probes used in this study (the actual diameters were always determined from the analysis of the SEM images). (b) Optical image registered when the probe shown in (a) established contact with ileum mucosa at a repulsive force of 10 nN. (c) Representative SEM image of the 15 µm colloidal probes used in the study. (d) Optical image registered when, while approaching ileum mucosa with the probe shown in (c), the signal registered by the PSD dropped to zero.