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. 2017 Aug 4;7:7299. doi: 10.1038/s41598-017-07561-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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1,25(OH)₂D₃ decreases TGF-β1/Smad signaling pathway via reducing Histone 3 acetylation in nasal polyp-derived fibroblasts. Nasal polyp-derived fibroblasts were treated with TGF-β1 and/or 1,25(OH)₂D₃ or 1,25(OH)₂D₃ alone or 4 hours. (A) Expression levels of acetylated-histone 3 were determined by Western blotting. Expression of histone 3 was utilized as the internal control. A representative experiment and quantitative determination of protein levels are shown. (B) The mRNA of COL1A1, COL1A2 and α-SMA promoter and its adjacent regions precipitated by acetyl-H3 were quantified by ChIP assay. All data are presented as mean ± SEM. Four primary cell lines from different donors were used. All experiments were performed in at least triplicate and repeated at least three times. *p < 0.05 vs. control, ^†p < 0.01 vs. TGF-β1.