Figure 5.

1,25(OH)₂D₃ decreases TGF-β1-induced collagen gel contraction and cell migration in nasal polyp-derived fibroblasts. Nasal polyp-derived fibroblasts were treated with TGF-β1 and/or 1,25(OH)₂D₃ for 72 hours. (A) Contractile activity was assessed by collagen gel contraction assay. These pictures show the results of one experiment; the contraction area was measured using an Image J analyzer. (B) Cell migration was assessed by Transwell migration assay. These pictures show one experimental result; cell number was measured using an Image J analyzer. All data are presented as mean ± SEM. Four primary cell lines from different donors were used. All experiments were performed in at least triplicate and repeated at least three times. *p < 0.05 vs. control, ^†p < 0.01 vs. TGF-β1.