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. 2017 Aug 4;7:7311. doi: 10.1038/s41598-017-07125-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of tight junction protein Claudin-5 is reduced in N-WASP^K14KO mice. Immunostaining of Claudin-5 (A) (n = 3) and E-cadherin (B) (n = 3) in N-WASP^K14KO and control mice skin showed reduced localization of Claudin-5 in N-WASP^K14KO but no significant change in E-cadherin localization. (C) Western blot analysis showed reduced Claudin-5 expression but no significant reduction in E-cadherin expression in N-WASP^K14KO compared to control mice skin (n = 3) (Blots were cropped and full length western blot images are in supplementary Fig. S6B).