Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 4;7:7305. doi: 10.1038/s41598-017-07848-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

PF-06747143 binds to CXCR4 in AML primary cells similarly to 12G5. (A) Representative histograms of flow cytometry comparing 12G5 (a commercially available CXCR4 Ab clone) and PF-06747143 CXCR4 antibody binding to patient 17 (P17^CXCR4-low) and patient 15 (P15^CXCR4-high) primary cells. These two patients were chosen for subsequent studies using PF-06747143. (B) Quantitative analyses of fluorescence intensity were performed on CD45⁺CD33⁺ cells of primary AML samples. The two patients chosen for subsequent studies using PF-06747143 were marked in red. Mean fluorescence intensity ratio (MFIR) for 12G5 and PF-06747143 were calculated by dividing the mean fluorescence intensity (MFI) of CXCR4 by the MFI of the respective nonspecific isotype control. (C) Correlation between 12G5 and PF-06747143 CXCR4 antibody binding to patient primary cells.