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. 2017 Aug 4;7:7335. doi: 10.1038/s41598-017-07777-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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BAPTA-AM treatment inhibits microtubule re-polymerisation. (a–c) Time-lapse images of U2OS cells expressing CENP-A-GFP and mCherry-α-tubulin during microtubule de-/re-polymerisation assay. Cells were released from nocodazole into fresh medium containing drugs as indicated. (d) A graph presenting the relative mCherry-α-tubulin at kinetochore of each treatment. Error bars indicate standard errors (n = 15 cells for control, n = 12 cells for BAPTA-AM, and n = 8 cells for BAPTA). Bar, 5 µm.