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. 2017 Aug 4;7:7335. doi: 10.1038/s41598-017-07777-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ca²⁺-depletion does not alter H3K9me3 and HP1α localisation. Fluorescence images of chromosome spreads prepared from control and BAPTA-AM treated cells for the localisation of H3K9me3 (a) and HP1α (b). Bar graphs below indicate H3K9me3 centromere-to-arm signal intensity (c) and HP1α/CREST signal intensity (d). Error bars indicate standard deviations derived from three independent experiments. Bar, 5 µm.