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. Author manuscript; available in PMC: 2017 Aug 5.
Published in final edited form as: Jpn J Ophthalmol. 2016 Jul 5;60(5):408–418. doi: 10.1007/s10384-016-0461-1

Fig. 5.

Fig. 5

Treatment with the exogenous recombinant protein TAT-HA-Prdx6 (for details on its construction, see section Prokaryotic expression of recombinant Prdx6 protein) attenuated UV-B-induced generation of ROS. hLECs were treated with 10 μg/mL TAT-HA-Prdx6 or TAT-HA-Prdx6 mutated at the active site (Mut) as the negative control, exposed to 2.0 (a) or 8.0 (b) kJ/m2 UV-B for 1 and 2 h, and processed for quantitation of ROS levels using the H2-DCF-DA dye at excitation and emission wavelengths of 485 nm and 530 nm, respectively. a Extrinsic supply of TAT-HA-Prdx6 significantly reduced ROS levels after 1 and 2 h of exposure to 2.0 kJ/m2 UV-B radiation (*P <0.0005, **P < 0.02, ***P < 0.00002, ****P < 0.003). b Extrinsic supply of TAT-HA-Prdx6 significantly reduced ROS levels after 1 h of exposure to 8.0 kJ/m2 UV-B radiation (*P <0.00002, **P < 0.0003, ***P < 0.035). Results are shown as the mean ± SD of three experiments. Asterisks denote significant differences