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Anti-nicotine antibody affinity estimated by competition ELISA. (A) Time-course affinity of anti-nicotine antibodies induced by various nicotine nanovaccines. (B) Endpoint comparison of antibody’s affinity among different hapten localization nanovaccine groups on day 40. Significantly different: * p < 0.05, ** p < 0.01, *** p < 0.001.
