Fig. 3.

Effect of PDN-SCFE on LPS-induced iNOS (a) mRNA and (b) protein expression in RAW 264.7 cells. Cells were pre-treated with indicated concentrations of PDN-SCFE for 2 h, followed by LPS for 6 h (mRNA expression) or 18 h (protein expression). The protein and mRNA levels of iNOS were determined by Immuno-blot and qRT-PCR methods, respectively. A representative blot from three separate experiments is shown here. The graphical figures represent the change in protein or mRNA expression normalized to actin. Data represent mean ± SD of three separate experiments. ^*** P < 0.001 vs control group; ^### P < 0.001 vs LPS-stimulated group