Table 1. Comparison of nDNA isolated from mouse liver by the phenol and NaI methodsa.
| µg DNA/g tissue | 260/280 ratio | oxo8dG/105dG | |||
| NaI |
Phenol |
NaI |
Phenol |
NaI |
Phenol |
| 488 |
350 |
1.89 |
1.85 |
0.033 |
3.623 |
| 316 |
290 |
1.92 |
1.83 |
0.039 |
2.822 |
| 387 |
434 |
1.93 |
1.86 |
0.031 |
3.475 |
| 465 |
368 |
1.89 |
1.88 |
0.038 |
3.019 |
| 269 |
482 |
1.90 |
1.91 |
0.030 |
3.248 |
| 366 |
302 |
1.85 |
1.81 |
0.040 |
2.681 |
| 378 |
322 |
1.86 |
1.87 |
0.033 |
3.644 |
| 434 |
500 |
1.86 |
1.83 |
0.029 |
3.308 |
| 510 |
494 |
1.88 |
1.78 |
0.035 |
2.779 |
| 297 |
280 |
1.86 |
1.80 |
0.038 |
3.700 |
| 391 ± 82 | 382 ± 88 | 1.88 ± 0.02 | 1.84 ± 0.02 | 0.035 ± 0.007 | 3.230 ± 0.069b |
aLiver tissue from 10 different mice was divided in half and nDNA was isolated from one half by the NaI method and from the other half by the phenol method. The nDNA was then hydrolyzed and analyzed using HPLC-EC as described in the Materials and Methods. The data for each liver sample is given as well as the mean ± SEM for the 10 animals.
bThis value is significantly (P < 0.001) greater than the value obtained by the NaI method as determined by the Student’s t-test.